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Research Progress in Preparation Technology of β-Nicotinamide Mononucleotide (NMN)

Release time: 2020-12-14

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Research Progress in Preparation Technology of β-Nicotinamide Mononucleotide (NMN)

  β-nicotinamide mononucleotide is a naturally occurring biologically active nucleotide, which can be synthesized in the human body, or obtained from daily vegetables and meat, and is closely related to human immunity and metabolism. In recent years, the anti-aging function of nicotinamide adenine dinucleotide (NAD+) has attracted widespread attention in the scientific community. A large number of animal experiments have shown that after increasing the content of NAD+, its aging organs can be restored to their youthful state. , And supplementation of β-nicotinamide mononucleotide (NMN) is a means to increase NAD+. At present, Japanese and American scholars have cooperated to conduct clinical research on NMN to investigate the safety and effectiveness of the substance on the human body. However, the current low output of NMN and high production cost limit its application.


  At present, the synthesis process of β-nicotinamide mononucleotide mainly includes chemical synthesis and biosynthesis. The price of biosynthetic enzyme is high, the source is limited, the production capacity of the biosynthetic process is low, and the production cost is high. As a result, the market price of NMN remains high.


  The chemical synthesis method has many steps and the yield is not high. The later purification is troublesome, and it is accompanied by the production of chiral isomers.


  The main biological and chemical preparation processes are now summarized as follows:


  Some chemical synthesis methods use nicotinamide ribose and diphenyl chlorophosphate to obtain β-nicotinamide nucleotides through esterification, and then hydrolysis and debenzene.


  There are also patents introduced: three-step synthesis method. Condensation reaction: ethyl nicotinate, tetraacetyl ribose and catalyst are condensed in a solvent to obtain ethyl nicotinate triacetyl nucleoside; ammonolysis reaction: add the condensation solution to ammonia solution to obtain nicotinamide nucleotide hydrochloride; phosphorus Chemical reaction: The salt obtained from the ammonolysis reaction is reacted with phosphorus oxychloride in a solvent to obtain nicotinamide mononucleotide.


  The method of enzymatic preparation of NMN is generally based on nicotinamide ribose as a substrate, in the presence of a phosphate group donor, under the catalysis of nicotinamide ribokinase/nicotinamide ribokinase-containing recombinant cells to produce β-nicotinamide mononucleoside acid. Enzymatic synthesis is generally environmentally friendly, with higher purity, and can be better applied in the fields of health care and biomedicine